(Munich)Microtubule

(Munich)Microtubule - thresholding the filter response is...

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Motivation Microtubules (MT) are filamentous cytoskeleton structures composed of tibulin protein subunits. Quantitative analysis of MT dynamics in live cell is necessary Growth and shortening events of MT by considering only the MT tip position is not a valid approximation if MT follow non-linear paths Reliable tracing of MT body is required
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Challenges Intersecting and overlapping MT regions appear brighter due to additive florescence Typical binarization methods yields gaps
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Tip Detection MT image is filtered by second derivative of Gaussian filter with different orientations to reveal curvilinear structures. Binary mask obtained by
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Unformatted text preview: thresholding the filter response is then further skeletonized to have B(x,y) The candidate tip positions are marked by finding the line ends in B(x,y) Methodology Use estimated tip positions as starting points Apply fast marching to extract all possible paths starting with tip position and end points s.t. Calculate the support of the path p Select the path that maximizes the support } , { y x tip tip tip = } 1 ) , ( , } , { : , { ) , ( = y x B N y x end end y x tip tip y x = p y x y x B s } , { ) , ( Results Results of the Geodesic Paths Results of the Proposed Algorithm...
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This note was uploaded on 12/28/2011 for the course BIO 100 taught by Professor Gomez during the Fall '11 term at UCSB.

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(Munich)Microtubule - thresholding the filter response is...

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