(Munich)nhatEM

(Munich)nhatEM - 3D Reconstruction of Anatomical Structures...

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3D Reconstruction of Anatomical Structures from Serial EM images
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Biological Motivation Reconstruct 3D structures from  serial stack of EM images to  understand Distribution of cell type and  organelles. Connectivity and vasculature. Requires  Tracing of cell membranes,  organelles, blood vessels, etc. Identification of structures for  serial correspondence. Currently, reconstruct only a  small fraction of volume (very  few objects). Time consuming (~20hours per  specimen). Wealth of information in  surround structures not utilized. Synapse Web, Kristen M. Harris, PI http://synapses.clm.utexas.edu/
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Serial TEM Dataset typical volume:  20-50 slices  8 x 5 x 0.05  m per slice 40 to 100  m 3  volume fly brain volume: 0.1mm 3 resolution:  xy: 2.6 nm/pixel (350~400  pixels/  m)  z: 0.05  m ~20 pixels apart storage size: small volume: ~100MB
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This note was uploaded on 12/28/2011 for the course BIO 100 taught by Professor Gomez during the Fall '11 term at UCSB.

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(Munich)nhatEM - 3D Reconstruction of Anatomical Structures...

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