Majumdar_ITRpresentation

Majumdar_ITRpresentation - STED:Nanoscale3DOpticalImaging

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STED: Nanoscale 3D Optical Imaging Department of Mechanical Engineering  Department of Materials Science University of California, Berkeley Materials Sciences Division Lawrence Berkeley National Laboratory
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Outline Motivation Introduction: Conventional Optics Near Field Imaging STED - Theory - Previous experimental work On-going experiments
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Virus Microtubules Cell Organelle Biological Imaging TMV 16.5 nm ER canaliculi dia. 40-60 nm Microtubule 25 nm
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Need for High Resolution Optical  Technique Biomolecules that require imaging are typically  1- 50 nm  in size Far-field optics  (e.g. confocal) limited to  resolution  200 nm which cannot directly resolve molecular-scale phenomena Atomic Force Microscopy  cannot be used  inside  a cell Optical/fluorescence   imaging is most-widely used       approach for  real-time intracellular  visualization NSOM  ( N ear-field  S canning  O ptical  M icroscope)
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Optical Imaging at a Glance Far-Field Optics Far-Field Optics Near-Field Scanning 
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Majumdar_ITRpresentation - STED:Nanoscale3DOpticalImaging

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