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111011cell-express-tobias

111011cell-express-tobias - ChE 170 Engineering Cell...

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Unformatted text preview: ChE 170: Engineering Cell Biology –The cell as a production factory, expression systems – 11/10/11 The Cell as a Production Factory, Expression Systems Tobias Schoep ChE 170: Engineering Cell Biology –The cell as a production factory, expression systems – 11/10/11 No chapter reference. Biology background, Chapter 15 Questions to [email protected], Rm 3114 or contact Serra Elliot ChE 170: Engineering Cell Biology –The cell as a production factory, expression systems – 11/10/11 ChE 170: Engineering Cell Biology –The cell as a production factory, expression systems – 11/10/11 Cells as production factories Cellular method of protein production depends on protein properties Different systems have different advantages and disadvantages for protein production To understand the advantages and disadvantages of expression systems we must understand a bit more biology Biology of protein folding, transport and modification – a brief overview Prokaryotic protein expression systems Eukaryotic protein expression systems ChE 170: Engineering Cell Biology –The cell as a production factory, expression systems – 11/10/11 Protein Production Prokaryotes : Translation in the cytoplasm Eukaryotes: Translation in the cytoplasm and endoplasmic reticulum ChE 170: Engineering Cell Biology –The cell as a production factory, expression systems – 11/10/11 Protein Folding (Eukaryotes) Proteins are produced in cytosol and endoplasmic reticulum Endoplasmic reticulum t argeting requires a signal sequence ChE 170: Engineering Cell Biology –The cell as a production factory, expression systems – 11/10/11 Protein Folding (Eukaryotes) Endoplasmic reticulm Function: Protein folding, glycosylation and export to golgi apparatus (eukaryotes) Correct folding eg. disulfide bond formation mediated by chaperones ChE 170: Engineering Cell Biology –The cell as a production factory, expression systems – 11/10/11 Protein Transport (Eukaryotes) Proteins move from ER to golgi apparatus in vesicles Golgi apparatus function: protein modification , packaging for distribution misfolded proteins ChE 170: Engineering Cell Biology –The cell as a production factory, expression systems – 11/10/11 Protein Transport (Eukaryotes) Lectures to come: Trafficking of proteins through cells Transport through membranes: facilitated and passive ChE 170: Engineering Cell Biology –The cell as a production factory, expression systems – 11/10/11 Protein Modification (Eukaryotes) Glycosylation Formation of protein / carbohydrate complex Important for protein structure, function and targeting Aberrant glycosylation can cause disease eg. Congenital Disorders of Glycosylation Many proteins glycosylated in ER ChE 170: Engineering Cell Biology –The cell as a production factory, expression systems – 11/10/11 Protein Modification (Eukaryotes) Glycosylation Additional modifications to glycoproteins in golgi apparatus ChE 170: Engineering Cell Biology –The cell as a production factory, expression...
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111011cell-express-tobias - ChE 170 Engineering Cell...

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