111311-hw2 f011

111311-hw2 f011 - ChE 170 Homework#2 Due Thurs:00 AM 1 You...

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ChE 170 Homework #2 Due Thurs Oct 20 11:00 AM 1. You are measuring the kinetics of an enzyme know to be present in an acidic cellular compartment involved in the degradation of proteins. When you isolate and purify the enzyme you discover that at 37°, pH = 7.2, the rate of product formation is 10-fold lower than that previously reported for the enzyme in its native environment. A. Write out a reaction mechanism (with arrows) that describes the most likely explanation for your slow kinetics. B.) Write out all of the equations necessary to derive an expression for the rate of product formation under the conditions above. C. Derive an expression for the rate of product formation. (show your work) 2. Derive an expression for the reaction velocity as a function of inhibitor concentration, substrate concentration for the case of mixed inhibition. Show all of your steps clearly. 3. Derive an expression for the effects of pH/proton concentration upon reaction velocity of lyozyme, assuming that there is one (rather than two) ionizable acidic residues in the
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This note was uploaded on 12/29/2011 for the course CHE 170 taught by Professor Ceweb during the Fall '10 term at UCSB.

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111311-hw2 f011 - ChE 170 Homework#2 Due Thurs:00 AM 1 You...

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