hw3-f011 102811

hw3-f011 102811 - ChE 170 F11 Homework 3 Due: Friday Nov 4,...

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ChE 170 F11 Homework 3 Due: Friday Nov 4, 4:00 PM (Box outside of TA office) 1. Affinity in oligomeric complexes. Histones involved in forming nucleosomes form a ring composed of eight monomers. a. Assuming each monomer has two binding sites that have equivalent affinity (K D = 10 M) for an identical histone monomer, calculate the concentration of each possible oligomeric species (e.g., H1,H2,…H8 ) present at equilibrium. Assume the nucleus is a sphere of diameter 4 microns, and that there are 10 7 octamer forming histones present in the nucleus. b. How would you expect the presence of double stranded DNA to influence or alter the ratios of the oligomer species? 2. For the two attached genes, identify the following elements in each of the DNA sequences below. a. ‐10 region b. ribosomal binding site c. Kozak sequence if present d. ‐35 region e. +1 site (start of mRNA transcript) f. TATA box if present g. Start codon h. Stop codon i. Transcription terminator j. INR sequence if present k. BRE sequence if present l. Is the gene from a procaryote or eucaryote? m. Using the genetic code, translate the DNA sequence into an amino acid sequence. 3.
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This note was uploaded on 12/29/2011 for the course CHE 170 taught by Professor Ceweb during the Fall '10 term at UCSB.

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hw3-f011 102811 - ChE 170 F11 Homework 3 Due: Friday Nov 4,...

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