HW5 - Department of Chemical Engineering University of...

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Department of Chemical Engineering ChE 170 University of California, Santa Barbara Fall 2010 Problem Set No. 5 Due: Thursday, 12/02/10 at the start of class Objective : To understand regulation and signaling processes in cells, and to develop and apply thermodynamic and kinetic models of them. Review problems You should pay special attention to these questions after reading. Note that the answers are given in the back of the book. Formulate your answers fully first and then check them. This can be a significant aid in your understanding of the material, and similar questions may be asked on the final. You do not need to provide written answers in the solutions you hand in. ECB 8-6 ECB 8-8 ECB 8-10 ECB 8-13 ECB 16-10 ECB 16-13 ECB 16-17 ECB 16-20 Problem 1 You are producing recombinant protein “X” in yeast cells. X is synthesized on the rough ER and secreted from the cell. You have a sensitive assay to measure the concentration of active protein in the growth medium in real-time. At the start of the experiment, the concentration of X throughout the cell is negligible. You initiate protein synthesis by addition of a chemical inducer that initiates transcription. Fluorescence experiments suggest that the concentration of X reaches 50% of its steady-state value in the ER in about 10 minutes, and in the Golgi apparatus in about 1 hour. How long do you expect it to take for the rate of secretion of X to the extracellular space to reach 90% of its steady-state value? Consider a two-compartment model of transport for this process (ER b Golgi), based on the one discussed in lecture.
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Problem 2 A DNA-binding regulatory protein g is synthesized on free ribosomes in the cytosol. The protein then must fold before it can be transported through the nuclear pore complex into the nucleus, where it binds to DNA. You will develop a simple kinetic model of this process considering the following steps: synthesis, folding, and transport through the pore complex. Consider first synthesis and folding in the cytosol.
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HW5 - Department of Chemical Engineering University of...

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