LAB 2.docx - 2.0 METHODS AND PROCEDURE 2.1 MATERIALS i...

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2.0 METHODS AND PROCEDURE 2.1 MATERIALS i. Compound microscope ii. Glass slides iii. Coverslip iv. Needle puncture v. Scissors vi. Hydrochloric acid vii. Watch glass viii. Bunsen burner ix. Clip x. Root tips of onion xi. Wright stain xii. Methylene blue 2.2 PROCEDURE A. Blood smear 1. Blood sample was taken by using a lancet to puncture the fingertip. The finger is pressed to draw a drop of blood. The lancet was disposed into the sharp bin afterwards. 2. The blood was smeared onto a clean glass slide. It was spread out with another slide to create a thin film of blood smear. 3. The Wright stain was used to stain the blood. It was left for 10 seconds. 4. The prepared slide of blood smear was placed under the microscope for observation. 5. The immersion oil was used to observe the slide. 6. Recordings of the observation was taken.
B. Cheek cell 1. The slide is cleaned using distilled water. 2. The cells were harvested using a toothpick and smeared onto the slide. 3. The methylene blue is used to stain the cheek cells. 4. The prepared specimen was covered with a coverslip and the excess water was dabbed away with a tissue paper. 5. The specimen was placed under the microscope for observation. C. Onion root tip 1. An onion root tip was cut in approximately 2-3 cm from its end.

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