Bi1x_2009_W3_S1

Bi1x_2009_W3_S1 - Page 1 of 2 Bi 1x, Spring 2009 Week 3,...

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: Page 1 of 2 Bi 1x, Spring 2009 Week 3, Session 1 PCR PCR In todays lab, you will use PCR to amplify a region of the 16S ribosomal RNA gene in the genomic samples you extracted in week 2. Materials: Sterile, ultrapure PCR-grade water 16S forward primer (F) 16S reverse primer (R) 2X PCR Master Mix Your purified bacterial DNA from last week PCR tubes Special handling notes:- All components of the PCR reaction must be kept on ice at all times. - Use only filtered pipet tips and change tips every time to avoid contamination. - Never mix your reactions by vortexing; only mix by pipetting up and down or flicking the tube gently. About the master mix: A PCR master mix is a concentrated solution of DNA polymerase, dNTPs (deoxynucleotide triphosphates: dATP, dTTP, dCTP, dGTP), and ions or additivesall the components required for PCR except DNA template and primers. Master mixes are more convenient than using separate components. The master mix you will be using today is Invitrogen Supermix II, which...
View Full Document

This document was uploaded on 01/03/2012.

Page1 / 2

Bi1x_2009_W3_S1 - Page 1 of 2 Bi 1x, Spring 2009 Week 3,...

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online