BindingFinal

BindingFinal - Lecture 4: Binding in Biology: A View...

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Unformatted text preview: Lecture 4: Binding in Biology: A View Lecture 4: Binding in Biology: A View From Statistical Mechanics From Statistical Mechanics The Biological Importance of Binding The Biological Importance of Binding ``Corpora non agunt nisi ligata’’ - ``A substance is not effective unless it is linked to another.’’ - Paul Ehrlich Biology is built around molecular recognition! Ligand Gated Channels The Immune System How Cells Decide The Role of Binding in the HIV Life The Role of Binding in the HIV Life Cycle Cycle Key point: One of the first processes in the infection process is the binding of the virus to the host cell. One class of measurements that characterize binding processes like this is the measurement of the ``affinity’’. From a conceptual point of view, we might want to know how other competitor molecules bind to the HIV gp120 in comparison with the CD4 receptor. How Are Measurements Made? How Are Measurements Made? Measure the heat released during binding reaction. Done by comparing how much energy needed to keep the temperature constant in the reactive chamber and in a reference chamber. Isothermal Titration Calorimetry Leavitt and Freire, 2001 What Is the Data Like? A Case Study What Is the Data Like? A Case Study from HIV from HIV CD4-gp120 thermodynamics. Calorimetry data for the titration of WD61 full-lengthCD4-gp120 thermodynamics....
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This document was uploaded on 01/03/2012.

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BindingFinal - Lecture 4: Binding in Biology: A View...

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