Single Molecule Bio Physics V2 - SLIDES

Single Molecule Bio Physics V2 - SLIDES - Single Molecule...

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Single Molecule Bio-Physics
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Single Molecule Fluorescence Techniques
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Single Molecule Fluorescence Techniques State of the Art imaging of single (immobilized) fluorescent Cy5 molecules Pictures: Tinnefeld Lab
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Fluorescence Techniques / GFP Green Fluorescent Protein (GFP) Discovered in Jelly Fish Nobel Prize 2008
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Super-Resolution Microscopy
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Super-Resolution Microscopy 4 s movie of actin labeled Cy5 molecules under 100 μM AA –O 2 1 ms integration time Real-time movie Analyzing frame by frame Pictures: Tinnefeld Lab
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Super-Resolution Microscopy J. Vogelsang, T. Cordes, C. Forthmann, C. Steinhauer, and P. Tinnefeld in PNAS 2009 , 106, 8107-8112 Pictures: Sauer Lab Actin Fibers stained with ATTO647
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Fret / Quenching
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Force Spectroscopy
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DNA Force Extension by Magnetic Tweezers Bustamante Lab
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Applying force to single molecules
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Molecular function of muscle Pictures: Gaub Lab
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Estimation of entropic forces on a polymer Pictures: Gaub Lab
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- ATP + ATP (2mM) Pictures: Gaub Lab
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Force Spectroscopy with Optical Tweezers
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This document was uploaded on 01/03/2012.

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Single Molecule Bio Physics V2 - SLIDES - Single Molecule...

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