Rate of things - spectroscopy POST

Rate of things - spectroscopy POST - Rate of things via...

Info iconThis preview shows pages 1–10. Sign up to view the full content.

View Full Document Right Arrow Icon
Rate of things via spectrophotometry Aph 162, Winter 2009 Week 2
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Overview Spectrophotometry The Beer-Lambert law Some weird units: OD 600 and cfu ’s – Calibration: a standard curve (OD 600 vs. cfu) Bacterial growth curves Growth on a single carbon source Growth on a two carbon sources ( diauxic growth/catabolite repression ) Experiments for today
Background image of page 2
Spectrophotometry: The Beer-Lambert law Relates concentration to the optical  measurement of ‘absorbance’ Example: E. coli concentration  Combined with spectrophotometry can be used  to distinguish and compare different molecules  in solution Example:  Chlorophyll spectrum
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Spectrophotometer
Background image of page 4
Spectrophotometer
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
z σ The Beer-Lambert law I = incident light ( W/cm^2)
Background image of page 6
z σ The Beer-Lambert law I = incident light ( W/cm^2)
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
z σ The Beer-Lambert law I = incident light ( W/cm^2)
Background image of page 8
z σ The Beer-Lambert law I = incident light ( W/cm^2)
Background image of page 9

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 10
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 26

Rate of things - spectroscopy POST - Rate of things via...

This preview shows document pages 1 - 10. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online