Bi1_2011_PS6_solution

Bi1_2011_PS6_solution - Bi1: The Biology and Biophysics of...

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Bi1: The Biology and Biophysics of Viruses Spring 2011 Problem Set 6: Viruses, ELISA and Antibodies Due Wednesday, May 18 at 4:00 P.M. in the Bi 1 closet Name: ANSWER KEY ___________________________________________________ Section # : ___________________________________________________________ Mail Code : ___________________________________________________________ TA Names : ___________________________________________________________ Date and Time turned in : _______________________________________________ Number of pages including this one : _____________________________________ AFTER YOU FINISH: How long did it take you to complete this problem set? ____________________________ Go to the Bi1 moodle site at http://courses.caltech.edu/ and take the homework survey There are 2 questions. The number of parts to each question is listed at the beginning of each; be sure to answer all the parts! Grade: Problem 1 __________ Problem 2 __________ TOTAL: _________ HOMEWORK INSTRUCTIONS 1) Turn in your homework stapled to this cover page. 2) Use separate sheets of paper for your answers. 3) Write or type your answers neatly. 4) Put your name on each page of your answers. 5) Box your answers, please, so that the grader can find them. Points may be deducted if you don’t follow these instructions!
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Page 2 of 14 Recommended reading for problem set: Chapter 49 (3/E and 2/E) with an emphasis on 49.2 (3/E and 2/E) Lectures 19 and 20 (Antibodies, Binding data, Flow cytometry) Problem 1: Gene delivery using a lentiviral vector and ELISA (60 points – 13 parts) Please read ALL text and figure legends carefully. You are interested in studying mutants of 2G12, a broadly neutralizing anti-HIV IgG antibody that binds a carbohydrate epitope on the HIV envelope protein gp120. To facilitate your research, you would like to create a cell line that stably expresses and secretes wild-type 2G12 (i.e., unaltered 2G12) and cell lines that stably express and secrete the different 2G12 mutants you will construct. Here we will guide you through the steps a researcher might take to create a stable cell line expressing a protein of interest. We will describe the procedure for wild-type 2G12 – the same procedure would be used to generate cell lines expressing each of the 2G12 mutants. Part I. A. (2 points) You have heard that some labs have used lentiviruses to deliver genes into cells. Give an example of a lentivirus and describe why lentiviruses might serve as useful vehicles for gene delivery. (≤ 3 sentences) Lentivirus is a type of retrovirus that is able to integrate its DNA into the host cell’s genome. It is able to replicate in non-dividing cells and can have long incubation periods. HIV is an example of a lentivirus. (Any accurate and specific definition of lentivirus and valid examples are acceptable). B.
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Bi1_2011_PS6_solution - Bi1: The Biology and Biophysics of...

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