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Bi1x_2009_W6

Bi1x_2009_W6 - Bi 1x Spring 2009 Week 6 Session 1 o Plate...

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Page 1 of 3 Bi 1x, Spring 2009 Week 6 Session 1 o Plate count anomaly Session 2 o Growth curves Session 1 – Plate count anomaly This "anomaly" was termed by Staley and Konopka in 1985 to describe the large discrepancy between the number of cells observed in natural environments by microscopy versus those that form colonies on solid media. During the Week 3 activities, you got an idea of the characters that inhabit the pond water through microscopy. Today, you will examine the pond bacteria by growing them on agar plates containing different media. Materials - Plates of different types; You will given a selection of the following: o Basal medium o Freshwater low nutrient medium o LB medium o Baxter pond water medium o BBB pond water medium o Beef broth medium - Liquid media to dilute with - Plating supplies Protocol 1. Create serial dilutions of the pond samples according to the instructions given by the TAs (total volume, 1 ml each dilution). To make the dilutions use the liquid form of the same type of media comprising your selected plates (for example, use LB to dilute if you are using LB plates).
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