Unformatted text preview: What do you expect to see in such a plot? Is there anything strange about your plots? Can you calculate a doubling time(s)? To find the relationship between OD and number of cells, use the provided data, from the LB + μ g/mL kanamycin. Knowing that the student plated 100 microliters on each plate, and the dilution factors, you should be able to find the number of cells per mL at both ODs. Plot these points and fit a line showing the OD-to-cell conversion factor. Is it close to the expected value? (OD 600 0.1=10 8 cells/mL) Discuss your error. Where might large errors have come from? Discuss the conclusions you can draw about challenges to E. coli with antibiotics and why kanamycin might differ from other antibiotics, like penicillin or vancomycin. How do these work? Can you estimate the MIC (minimum inhibitory concentration)? What is the clinical significance of the MIC and what can doses of antibiotic below that level lead to in the long run? Can you find an example of this?...
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- Fall '09
- Bacteria, kanamycin, minimum inhibitory concentration, OD-to-cell conversion factor