Unformatted text preview: o Chicken broth medium o Carrot medium, with and without vitamin B12 o Egg plates o Sports-drink plates -Liquid to dilute with -Plating supplies Protocol 1. Create serial dilutions of the pond samples according to the instructions given by the TAs (total volume, 1 ml each dilution). 2. Spread 100 µ l of each dilution on a separate plate using plating beads. 3. Label your plates and place them upside down on your bench to incubate. What do you expect to grow on the plates? Will there be differences between the ponds? with and without glucose? with and without B12? Why are we using chicken broth? What’s in it that’s interesting? Why are we using eggs? What nutrients does it supply that the others don’t?...
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This document was uploaded on 01/03/2012.
- Fall '09