Unformatted text preview: A TA will help you load and start the centrifuge. Afterwards, remove the supernatant, leaving just the pellet. (A muddy pellet is ok.) A macropipettor is useful here. PowerSoil Kit Prep Remove the beads and solution from PowerBead Tube and transfer into another sterile microcentrifuge tube. Add 0.25g of your wet soil sample to PowerBead Tube and centrifuge at room temperature for 30 seconds at 10,000 x g . Remove as much liquid as possible with a pipet. Add beads and bead solution back to PowerBead Tube and place on ice. These will be frozen and saved for the protocol tomorrow....
View Full Document
- Fall '09
- 2000s music groups, 1990s music groups, The Tubes, wet soil sample, PowerBead Tube