Week 4_handout

Week 4_handout - Bi 1x Spring 2010 Week 4 Session 2 o...

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
Bi 1x, Spring 2010 Week 4 Session 2 o Restriction enzyme digestion Restriction enzyme digestion Restriction enzymes are enzymes that bind to specific DNA sequences and cleave (“digest”) the DNA at or next to the binding site. You will become familiar with restriction enzymes during this week’s lab sessions on both bulk and single molecule scales. Note: This document describes bulk restriction digests only. Materials: Sterile, ultrapure water pZE21-lacZ plasmid DNA 10X NEB2 Buffer 10X EcoRI Buffer 10X BSA Solution HindIII digested lambda phage DNA EcoRI, HindIII, and KpnI restriction enzymes will be supplied by your TAs when you are ready to use them. Background: Most useful restriction enzymes recognize 4-8 base pair restriction sites. These sites are symmetric, inverted repeats called palindromes. Shown below are the restriction sites of the three enzymes you will be using today: EcoRI, HindIII, and KpnI. Notice how the 5’ to 3’ sequence is identical on the top and bottom strands.
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

This document was uploaded on 01/03/2012.

Page1 / 3

Week 4_handout - Bi 1x Spring 2010 Week 4 Session 2 o...

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online