Group-2 paper presentation

Group-2 paper presentation - A Presentation by Team...

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Unformatted text preview: A Presentation by Team Dope (Lindsay King, Micah Solomon, Ronnie Bryan) ¡ Reward without Dopamine The Journal of Neuroscience , 2003 Claire Matson Cannon and Richard D. Palmiter ¡ Morphine reward in dopamine-deficient mice Nature , 2005 Thomas S. Hnasko, Bethany N. Sotak, & Richard D. Palmiter ¡ Fact: Drugs of abuse and natural rewards activate dopaminergic systems ¡ Question: Is dopamine necessary for reward? ¡ How would YOU address this question, class? Dopamine Niiiice …. I liiike it! Substance X ? ? ? ? ¡ Dopamine-deficient mice (DD) ¡ DD mice lack tyrosine hydroxylase (TH) ¡ TH catalyzes the conversion of tyrosine to DOPA, which is a precursor to dopamine ¡ What might be the effects of this manipulation, class? tyrosine DOPA dopamine TH DDC ¡ Dopamine is a precursor to Noradrenaline and adrenaline ¡ How can we avoid losing noradrenergic and adrenergic activity? ¡ Dopamine is a precursor to Noradrenaline and adrenaline ¡ How can we avoid losing noradrenergic and adrenergic activity? ¡ Add transgene TH to DBH promotor! ¡ Wait, what’s DBH? PNMT DBH TH Normal pathway DDC ¡ In DD mice, TH is missing, but TH activity is restored in NA neurons via insertion into the DBH promoter Dopamine Neuron – TH is not created because DBH is never expressed Adrenergic Neuron – TH is created whenever DBH is expressed TH DBH TH DDC Time (hrs) Time (hrs) [DA] Feeding 18 hrs L-dopa injection ¡ Dopamine-deficient mice have issues ¡ Are “runted, hypoactive, and hypophagic” ¡ must be administered L- dopa daily to restore normal feeding behavior ¡ After 18hrs, DA is ~1% of normal ¡ Schematic data to left illustrates this L-dopa injection ¡ How do we quantitatively assess reward preference in mice? ¡ What kind of reward? ¡ By using a lick-o-meter! ¡ Measure the licking preference between 2 tubes: one with sugar water and one with regular old water – # of licks and bout length (lick rate) lick-o-meter, artist rendition Why use this paradigm instead of bar- pressing, maze-running, or condition place preference, class? ¡ By using a lick-o-meter!...
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Group-2 paper presentation - A Presentation by Team...

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