Group-2 paper presentation

Group-2 paper presentation - A Presentation by Team Dope...

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Unformatted text preview: A Presentation by Team Dope (Lindsay King, Micah Solomon, Ronnie Bryan) Reward without Dopamine The Journal of Neuroscience , 2003 Claire Matson Cannon and Richard D. Palmiter Morphine reward in dopamine-deficient mice Nature , 2005 Thomas S. Hnasko, Bethany N. Sotak, & Richard D. Palmiter Fact: Drugs of abuse and natural rewards activate dopaminergic systems Question: Is dopamine necessary for reward? How would YOU address this question, class? Dopamine Niiiice . I liiike it! Substance X ? ? ? ? Dopamine-deficient mice (DD) DD mice lack tyrosine hydroxylase (TH) TH catalyzes the conversion of tyrosine to DOPA, which is a precursor to dopamine What might be the effects of this manipulation, class? tyrosine DOPA dopamine TH DDC Dopamine is a precursor to Noradrenaline and adrenaline How can we avoid losing noradrenergic and adrenergic activity? Dopamine is a precursor to Noradrenaline and adrenaline How can we avoid losing noradrenergic and adrenergic activity? Add transgene TH to DBH promotor! Wait, whats DBH? PNMT DBH TH Normal pathway DDC In DD mice, TH is missing, but TH activity is restored in NA neurons via insertion into the DBH promoter Dopamine Neuron TH is not created because DBH is never expressed Adrenergic Neuron TH is created whenever DBH is expressed TH DBH TH DDC Time (hrs) Time (hrs) [DA] Feeding 18 hrs L-dopa injection Dopamine-deficient mice have issues Are runted, hypoactive, and hypophagic must be administered L- dopa daily to restore normal feeding behavior After 18hrs, DA is ~1% of normal Schematic data to left illustrates this L-dopa injection How do we quantitatively assess reward preference in mice? What kind of reward? By using a lick-o-meter! Measure the licking preference between 2 tubes: one with sugar water and one with regular old water # of licks and bout length (lick rate) lick-o-meter, artist rendition Why use this paradigm instead of bar- pressing, maze-running, or condition place preference, class? By using a lick-o-meter!...
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Group-2 paper presentation - A Presentation by Team Dope...

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