Bi114 Problem Set 1_012011_updated

Bi114 Problem Set 1_012011_updated - Bi114 Problem Set 1...

Info icon This preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
Bi114 Problem Set 1 Due by 5pm, Thursday, January 27th Problems sets may be dropped off at Church 275 or emailed to [email protected] 1. Read Blander & Medzhitov 2006 Nature a) What does the localization of I-A b indicate (e.g. Fig. 1a vs 1b)? Please explain. b) In Fig. 3, what evidence did the authors provide to demonstrate that an antigen needs to be in the same cargo with a TLR ligand (e.g. LPS) to elicit antigen-specific immune response? Based on the data in Fig. 3, please explain why in Fig. 2a, co-stimulation of EAP + apoptotic cells and OVA + E. coli leads to the presentation of only OVA but not EAP. c) What does the Ii degradation assay measure (e.g. Fig. 4a)? Please explain. d) A mouse was sacrificed. Bone marrow was washed out of the bones by needle aspiration. Cells were cultured for ten days with GM-CSF. By day 10, 90% of cells in culture were mouse dendritic cells. A fraction of the cells were lysed using a protein lysis buffer.
Image of page 1
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern