BMB170a_2011_LECTURE9

BMB170a_2011_LECTURE9 -...

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!"! $%&' ()*+,-) . /,*0)1* 2*1345 6*+ 785 7&$$ /,*0)1* 2*134 9 :'41* 1;+-<3,*=<; /,*0)1* 2*13 >'+':'4) !)-?'; !" $% @$..7A BCD) /,*0)1* 2*13 >'+':'4)E 2 F<?G-)D);41H) I)0'=<;'0 >'+':'4) <J CD-))K>1?);41<;'0 L+-,*+,-)4 <J /,*0)1* 2*134M !1<GDN4 O PQE%8$ DRGESS;3:4)-H)-T-,+U)-4T)3,S
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Phage discovery Federick Twort (1915) V2; 1;H)4=U'=<; <; +D) ;'+,-) <J ,0+-'K?1*-<4*<G1* H1-,4)4V (';*)+ $WPE$7X$KQ Félix d'Herelle “An invisible microbe that is antagonistic to the dysentery bacillus” (1917) Compt R Acad Sciences 165:373-5 “phages are found where bacteria thrive” Total ! 10 30 particles (aver MW ~150x 10 6 ) Biomass 2.5 x 10 11 kg! (Human ~4 x 10 8 kg) Ocean is most prevalent Gene transfer events (random packaging) ~2 x 10 15 per sec (lab conditions) Some density comparison in the ocean bacteriophage ~50,000,000/ml (10 7 ) sharks << 0.000001/ml (10 -6 ) Soil 10 7 /g with root associated Ruminants 10 7 /g of feces ~ 40% of bacterial background infected everyday YD'U) @ '; )'+)-A Pedulla et al Cell (2003) 113:171-82 Mycobacteriophage
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YD'U) 4+-,*+,-) I';U) 1; 41Z) J-<? 7XK7&&;? 1; 0);U+D [)'3 *'; :) 1*<4'D)3-'0 <- \0'?);+<,4 "';N D'H) +'104 -)4G<;41:0) J<- 'R'+*D?);+ ';3 >/2 1;])*=<; Baker Lab: Tao et al Cell (1998) 95:431-7 T4 P22
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DNA packaging by phage Ecoli particle attacked by T4 Phage (X25K) DNA is the genetic material “Hershey/Chase” radioactively label RNA or protein infect bacteria and shear off particles where is the radioactivity? DNA is packed tight Phillips analogy “Put 500m of Golden Gate Bridge suspension cable in the back of a FedEx truck.
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