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BMB170c_2011_PS2 - BMB/Bi/Ch 170c 2011 Problem Set 2...

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BMB/Bi/Ch 170c 2011 Problem Set 2: Chaperone Due: 4/21/2011 Disulfide bond formation (Bardwell et al) a. What initial evidence is given to suggest that disulfide bond formation needed to be facilitated by a protein factor in vivo? Why do the authors believe that DsbA is involved in disulfide bond formation? b. In order to determine the role of DsbA in disulfide bond formation, the authors needed a method to measure said disulfide bond at varying time points. What experimental techniques are used for such role? What were the findings of the experiment? c. How do the authors determine that any retardation in disulfide bond formation is not a result of other processes? d. The authors determine that DsbA is non-essential to bacterial growth, yet their experiments with the dsbA::kan1 mutant show defects in disulfide bond formation. How do you rationalize this difference? Hsp104, Hsp70, Hsp40 chaperone system (Glover & Lindquist) a. This paper identifies the role of an Hsp104 chaperone system. How does the Hsp104 system differ from other chaperone systems (such as GroEL, DnaK, Ssa1/Ydj1)?
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