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Unformatted text preview: BMB/Bi/Ch 170c 2011 Problem Set 3: Viruses/HIV Due: 5/3/2011 1. Atomic-level modeling of the HIV capsid (Pornillos et al). a. Describe the structure of the HIV capsid and the interactions in which CTD and NTD of the CA monomer are involved. (10 points). b. In both the CA hexamer and the CA pentamer, monomers interact via the same surfaces. What feature(s) of the CA protein make(s) this possible? (5 points). c. Explain why the pentamers composed of wild-type CA are observed in the HIV capsid despite their low stability compared to the hexamers. (5 points). d. Briefly comment on the significance of this study. How does modeling of the HIV capsid reconcile two different crystal structures of the CTD dimer mentioned in the paper? (5 points). e. Briefly describe the crystallization procedure used in this study. What steps did the authors take to obtain the CA pentamers? What are the R work factors and what do they suggest about the crystallographic models? (5 points)....
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- Fall '09