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Chem 24 FRET Lecture

Chem 24 FRET Lecture - Fluorescence Spectroscopy FRET...

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Fluorescence Spectroscopy & FRET absorption fluorescence This energy difference is called the Stokes shift
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What do these Stokes shifts tell you about the nature of the absorption Fluorescence Spectroscopy & FRET
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Fluorescence Spectroscopy & FRET another form of the Jablonski plot electrons in the S1 state can undergo spin conversion if there is overlap in the excited state energies can be associated with solvent relaxation (10 -10 to 10 -12 sec) relaxation of a polar solvent increases the Stokes shift (lowers the energy of the T 1 state)
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