cells_microscopy-lab

Cells_microscopy-lab - Cells page 1 of 5 ANAT 11110 Fall 2011 Lab 1 The Cellular Level of Organization The cell is the structural and functional

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Cells page 1 of 5 ANAT 11110 Lab 1 Fall 2011 The Cellular Level of Organization The cell is the structural and functional unit of all living things. The cells of the human body are very diverse; their size and shape vary tremendously and are tailored to the function they perform in the body. However different they are, most cells contain many structures and components in common. In this lab we will be looking at some of the elements which make up a cell. To do this, we will use one of the most pervasive tools in anatomical investigations: the microscope. The microscope allows us to look at anatomical structures too small to be viewed by the eye alone. This lab will therefore first acquaint you with the proper use of the microscope. Station 1: The Compound Microscope In order to use a microscope properly, it is necessary to learn the names and functions of each part. Refer to the microscope and the diagram as you go through the definitions given below: -ocular (aka, eyepiece) : the lens (or lenses) that you look through; usually 10X or 15X (‘X’ means ‘times the actual size’) -objective(s) : the lens immediately next to the specimen to be looked at; usually there will be 3 or 4 objective lenses, in varying magnifications (typically 4X, 10X, 40X, and 100X); generally the higher the magnification, the longer the lens. To calculate the total magnification on a particular setting, multiply the power of the ocular lens times the power of the objective lens (e.g., 10X [ocular lens] times 40X [objective lens] equals a total magnification of 400X). -revolving nosepiece (aka, turret) : holds the objective lenses; can be rotated to position the desired lens into place -tube : connects the ocular to the objective lenses -arm : supports the tube and connects it to the base -base : the bottom of the microscope, used for support -stage : the flat platform where the slide is placed; there is usually an arrangement of clips or clamps to hold the slide in place; make sure the slide is secure and sitting flat against the surface of the stage. If the microscope has a ‘mechanical stage’, you will be able to move the slide (left, right, forward, backward) by turning two knobs. -coarse and fine adjustment knobs : used to bring the specimen into focus; start with the coarse adjustment knob and then refine the image by using the fine adjustment knob -condenser lens and adjustment knob : found immediately below the stage; serves to focus light on the specimen; adjusted by turning an adjustment knob or moving a lever; in some microscopes, the position of the condenser is fixed.
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This note was uploaded on 01/03/2012 for the course ANAT 11110 taught by Professor Manley-buser during the Fall '11 term at Palmer Chiropractic.

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Cells_microscopy-lab - Cells page 1 of 5 ANAT 11110 Fall 2011 Lab 1 The Cellular Level of Organization The cell is the structural and functional

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