17 - LECTURE 17 ANNOUNCEMENTS 1. ASSIGNMENTS FOR THIS WEEK...

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LECTURE 17 ANNOUNCEMENTS 1. ASSIGNMENTS FOR THIS WEEK AS IN LECTURE GUIDE FOR WEEK STARTING 10/3. 2. PyMOL # 6, CHYMOTRYPSIN: THE ONE AND ONLY PyMOL ASSIGNMENT TO APPEAR ON THE MIDTERM EXAM. ** NO OFFICE HRS OR REVIEWS FOR THIS PyMOL UNTIL SUN 10/16! 3. CONFLICT FOR MIDTERM EXAM Th 10/20 7:30PM? E-MAIL PROF BEFORE FALL BREAK TO DESCRIBE CONFLICT . MEET BRIEFLY AFTER CLASS ON WED 10/5 TO SET THE DAY AND TIME . PERHAPS SAT 10/22 OR SUN 10/23? FRIDAY S LECTURE TODAY HOW TO COMPARE "EFFICIENCY" OF DIFFERENT ENZYMES IRREVERSIBLE INHIBITION USE OF pH TO LEARN ABOUT IONIZABLE RESIDUES AT THE ACTIVE SITE COMPETITIVE AND NON-COMPETITIVE INHIBITION CHYMOTRYPSIN MECHANISM, STEP-BY-STEP
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INTERPRETATION OF THE DIAGRAMS OF MECHANISM: 1. STRUCTURES DRAWN ON THE "ENZ CIRCLES" ARE SUBSTRATE, INTERMEDIATES, PRODUCTS, AND SOME AA OF CHYMOTRYPSIN 2. NUMBERED ARROWS DRAWN BETWEEN "ENZ CIRCLES" CORRESPOND TO RXN STEPS G o E+S ES' ES'' + P 1 ES''+ BOUND WATER ES''' EP 2 E+P 2 TS ES A REAL ENZYME MECHANISM! NOT: E + S ES E + P A SNAPSHOT A PROCESS
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THE PROBLEM OF SHOWING 3D IN 2D POLYPEPTIDE SUBSTRATE 2D PICTURE TO SHOW "CHEMISTRY" OF ELECTRON FLOW MORE REALISTIC PSEUDO 3D PICTURE OF BOUND SUBSTRATE AND PART OF CATALYTIC TRIAD POLYPEPTIDE SUBSTRATE OXYANION HOLE Ser 195 Asp 194 Gly 193
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CHYMOTRYPSIN free enzyme ES COMPLEX SHORT-LIVED INTERMEDIATE SHORT-LIVED INTERMEDIATE EACH OF THE DRAWN STRUCTURES EXISTS HAS SOME DEGREE OF THERMODYNAMIC STABILITY. i.e. BETWEEN THESE STRUCTURES: CATALYSIS! EP COMPLEX G o BUT BETWEEN THESE STRUCTURES ARE TRANSITION STATES! OVERALL MECHANISM OF CHYMOTRYPSIN-CATALYZED PEPTIDE HYDROLYSIS CONVENTION: CURVED ARROWS OF e - FLOW ON EACH FIGURE SHOW WHAT OCCURS DURING STEP SHOWN BY THE NUMBERED STRAIGHT ARROW
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This note was uploaded on 01/01/2012 for the course BIOMG 3310 taught by Professor Feigenson during the Fall '11 term at Cornell University (Engineering School).

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17 - LECTURE 17 ANNOUNCEMENTS 1. ASSIGNMENTS FOR THIS WEEK...

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