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Unformatted text preview: Net ID __________ BIOMG 3310 Quiz 3a 9/22/10 1. (12 pts) Consider the experiment of the steps of unfolding, then re-folding the small protein actin. This protein has no disulfide bonds. Draw the indicated graphs. N = native structure a. the protein under physiological conditions b. the protein, but in 8M urea c. the protein in 8M urea, but with mercaptoethanol (HSCH 2 CH 2 OH), a disulfide reducing agent, added d. after step (b), then dialyzing to remove the urea 2. (6 pts) Consider the general nature of the process, FOLDED PROTEIN UNFOLDED PROTEIN (under physiological conditions). Answer - if negative or + if positive for (a - e) a. ∆ G o for the unfolding process ____ b. ∆ H o for the unfolding process ____ c. T ∆ S o for the unfolding process ____ d. T ∆ S o for unfolding the protein only (not including the water) ____ e. T ∆ S o for the water only when the protein unfolds (not including the protein) ____ f. For a large, multidomain protein that misfolds, what kind of protein in the cell would give it a second chance to fold correctly? __________________ 3. (8 pts) Calculate the magnitude of the free energy change by which prolyl isomerase lowers the barrier for cis ↔ trans interchange, given that: (RATE cat / RATE uncat ) = (exp- ∆ G o ± /RT ) cat / (exp- ∆ G o ± /RT ) uncat R = 0.00832kJ/mol x K and T = 300K RATE cat /RATE uncat = 1000....
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- Fall '11