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Lab 3 Osmosis and Diffusion Procedures

Lab 3 Osmosis and Diffusion Procedures - 1 Each group...

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Drew Rasmussen Penny Louka Biology 191 Lab September 7 th , 2010 Lab 3: Diffusion, Osmosis, and Membranes: Procedures Procedure 1: Does Molecular Mass Affect The Rate of Diffusion What do you hypothesize: I believe molecular mass will affect the rate of dffusion because the heavier the particle, the harder it will be to get it moving due to inertia. 1. Your instructor will inoculate small amounts of potassium dichromate (molecular mass 294), Janus green B (molecular mass = 511), and malachite green (molecular mass 929) about 5 cm apart in the surface of an agar plate. 2. At the end of class, measure the distance traveled by each type of molecule a. Distance moved by potassium dichromate: 1.5cm b. Distance moved by Janus green B: 1cm c. Distance moved by malachite green: 0.8cm d. This data is consistent with my hypothesis, the heavier liquids diffused much less. Procedure 4: How Could You Determine The Solute Concentration of a Plant Cell?
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Unformatted text preview: 1. Each group should cut size pieces of celery, each about 3 cm long. Weigh each piece, and record its mass in Table 2. 2. Obtain six paper clips of the same color (each group will have a different color clip). Attach a clip to each of your celery pieces, keeping track of which piece will go into which solution. 3. Place one piece in each of the six provided salt solutions, ranging from 0% to 12% salt. 4. After one half hour, remove your celery pieces, weigh them and record the masses in table 2. 5. Create a graph of percent mass change on the y-axis and salt concentration on the x-axis. The salt concentration at which 0% mass change occurs corresponds to the solute concentration of the celery cells. In other words, look for the x-intercept (where percent mass change is zero). 6. What is the approximate solute concentration in the celery cells? ______________...
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