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1 postmed_intro2007_mod_nxpowerlite_

1 postmed_intro2007_mod_nxpowerlite_ - MEDICAL CELL AND...

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MEDICAL CELL AND TISSUE BIOLOGY (BMS 6110C) INTRODUCTION Dr. Kelly Selman [email protected] August 20, 2007 Reading Assignment: Laboratory Manual: Introductory material Ross & Pawlina 5 th Ed., Chapter 1
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Objectives Overview of Medical Cell & Tissue Biology Terminology Techniques Microscopes Tissue preparation Stains Muscular dystrophies
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Skin, H&E
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* Cell 1 Cell 2 Cell 1 Cell 2 Transmission electron microscope Light microscope Light microscope .
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Units of Measurement Micrometer (µm): 10 -3 mm Nanometer (nm): 10 –6 mm = 10 -3 (µm) Angstrom (Å): 10 –7 mm = 10 -4 (µm) = 10 –1 nm
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Size Relationships * Cell membrane ~7.5 nm *
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Why use a microscope? Magnification Linear enlargement of an object Ratio of image size to object size Resolution (resolving power) Measurement of ability to distinguish 2 closely positioned points as 2 distinct entities Property of the objective lens of LM and λ of light R= 0.61 λ N.A. of objective lens Resolution of LM: 0.2 µm
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Empty magnification
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Resolution Eye: 0.2 mm Light microscope (LM): 0.2 µm Scanning electron microscope (SEM): 2.5 nm Transmission electron microscope (TEM): 0.05-1.0 nm
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Light microscope (LM) (Bright-field microscope) R: 0.2 µm Gall Bladder, H&E
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Bright-field and Fluorescence Microscope Fluorescence: property of absorbing light at one λ (uv, short) and emitting light at another λ (longer, visible light)
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Transmission Electron Microscope (TEM) Resolution: 0.05-1.0 nm) Electron micrographs
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Transmission Electron Microscope (TEM) Better resolution (0.05-1.0 nm) Higher magnification See more cellular detail Can not examine live tissue Special preparations Very thin sections No color dyes Projector lens Phosphorescent screen Condenser lens Specimen Objective lens Cathode Emits electrons
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LM TEM Skeletal muscle
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TEM Small intestine LM
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(0.2 µm) (0.05-1.0 nm) ( 2.5 nm) SEM LM TEM Computer screen
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SEM LM Oviduct Cilia Cilia
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Preparation of Specimens for Microscopy (LM) (TEM) colored sections sections (retains phospholipids)
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Preparation of sections for LM & TEM
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Why stain sections?
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