17 cytoskeleton_07_aris

17 cytoskeleton_07_aris - Medical Cell and Tissue Biology...

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Medical Cell and Tissue Biology BMS 6110 John P. Aris, Ph.D. Rm. B1-8, 392-1873, johnaris@ufl.edu Reading: Cytoskeleton
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Cytoskeleton Eukaryotic cells have a cytoskeleton composed of different types of protein filaments The cytoskeleton is primarily cytosolic (the nuclear lamina is an exception) Micrograph of a tissue culture cell stained with Coomassie blue dye.
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Cytoskeleton Cytoskeletal fibers do not pass through membranes (e.g., cytokeratin fibers pass around the nucleus) Immunofluorescence micrograph showing immunostaining of cytokeratin fibers in human keratinocytes (green fluorescence) Nuclei are stained with DNA-specific dye (blue fluorescence)
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Collagen Fibers in the ECM Collagen type I fibrils in the ECM are usually 50-100 nm in diameter and are wider than cytoskeletal fibers in the cytosol Collagen type I fibrils
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Cytoskeletal Proteins Cytoskeletal proteins are made by free ribosomes
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Cytoskeleton Functions Structural Mechanical support for cells and tissues Internal framework for maintaining positions of organelles Force-Generation Mechanism for movement of internal parts (e.g., vesicles) Mediates changes in cell shape (e.g., phagocytosis, cell movement, and cytokinesis) Separation of chromosomes during mitosis
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Cytoskeletal Filaments and Tubules Type Diameter Protein Microfilaments ~7 nm Actin Intermediate filaments ~10 nm IF proteins Microtubules 25 nm Tubulin
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Cytoskeleton Distribution Microfilaments Microtubules Intermediate Filaments Immunocytological staining of different cytoskeletal fibers in the same cell IFs, MTs, and MFs may have overlapping distribution in cell
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Cytoskeleton in Epithelium IFs, MTs, and MFs may have specific distribution in cells IFs and MFs associate with cell-cell and cell-matrix junctions
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Intermediate Filaments Present in all multicellular (metazoan) organisms Solid filament with diameter of ~10 nm, which is “intermediate” between MFs and MTs
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This note was uploaded on 01/04/2012 for the course ANT 610C taught by Professor Johnaris during the Spring '10 term at University of Florida.

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17 cytoskeleton_07_aris - Medical Cell and Tissue Biology...

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