21 blood_hematopoiesis_07_nxpowerlite_

21 - Medical Cell and Tissue Biology BMS 6110 John P Aris PhD Rm B1-8 392-1873 [email protected] Reading Ross& Pawlina 5 th Ed Ch 10 Blood&

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Unformatted text preview: Medical Cell and Tissue Biology BMS 6110 John P. Aris, PhD Rm. B1-8, 392-1873, [email protected] Reading: Ross & Pawlina, 5 th Ed, Ch 10 Blood & Hematopoiesis Blood Specialized connective tissue Composition 1. Plasma 2. Cells A. Erythrocytes B. Leukocytes i. Agranulocytes ii. Granulocytes 3. Platelets Blood Functions 1. Transport A. Gases (e.g., O 2 , CO 2 ) B. Nutrients and waste products C. Regulatory molecules (e.g., hormones) 2. Homeostasis A. Thermoregulation 3. Protection A. Vascular integrity (e.g., coagulation) B. Immune cells and molecules (e.g., IgGs) Plasma Water ~91% Proteins ~7% Albumin, globulins, fibrinogen Ions ~1% Bicarbonate (21-25 mM) Calcium (2.2-2.7 mM) Chloride (94-111 mM) Potassium (4.1-5.6 mM) Sodium (137-148 mM) Small Molecules ~1% Glucose (3.4-5.6 mM) Lactate (1.0-1.8 mM) Urea (3.4-6.7 mM) Lipids <1% Cholesterol (3.4-5.2 mM) Fatty acids (free) (0.2-0.9 mM) Triglycerides (0.4-15 g/L) Blood Cells and Platelets RBCs - constitute >99% of blood cells by number Hematocrit - packed RBC volume (% of blood volume) Lifespan - Neutrophils, only 12-14 hours Platelets, ~10 days RBCs, ~4 months (~120 days) Lymphocytes, many years Cell % of volume # per microliter (µl) Erythrocytes ~40 - 50% (M) ~35 - 45% (F) 4.3 - 5.7 million (M) 3.9 - 5.0 million (F) Leukocytes ~1% 3.5 - 10.5 thousand Platelets <1% 150 - 450 thousand Erythrocytes • Transports O 2 (bound to hemoglobin) • Converts CO 2 into HCO 3- (transported in plasma) • Biconcave disk-shape, deformable, resilient, no organelles • Plasma membrane and spectrin cytoskeleton present • Proteins - mainly hemoglobin ( α 2 β 2 ) and glycolytic enzymes • Quality control - spleen removes old or damaged RBCs RBC Plasma Membrane Skeleton Resilient and flexible shape of RBC is due to spectrin- based membrane skeleton linked to cytoplasmic side of the plasma membrane Plasma membrane Freeze-fracture EM of RBC Membrane 1. Freeze-fracture Freeze and fracture Exoplasmic face Cytoplasmic face 2. Prepare replica Produce carbon replica Shadow with platinum Dissolve sample View replica in EM Low mag High mag Band 3 particles 3. View in TEM Freeze-fracture EM shows that 'Band 3' protein spans membrane Leukocytes Granulocytes Neutrophil (E) Band Neutro. (I) Eosinophil (D) Basophil (J) Agranulocytes Monocyte (F) Lymphocyte (H) Platelets (G) Leukocytes Size- larger than erythrocytes (10 - 18 µm diameter) All- contain non-specific azurophilic (primary) granules that are equivalent to lysosomes Granulocytes- contain specific (secondary) granules that contain specific enzymes for each cell type Agranulocytes- no specific granules present Granulocyte Neutrophil ~60% Eosinophil ~4% Basophil <1% Agranulocyte Lymphocyte (T, B, NK) ~26% Monocyte ~9% Leukocytes Distribution- blood and CT (as transient or wandering cells) Function- immune protection, movement (cell motility) Diapedesis- movement out of blood into connective tissue Chemotaxis- movement directed by homing molecules Neutrophil...
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This note was uploaded on 01/04/2012 for the course ANT 610C taught by Professor Johnaris during the Spring '10 term at University of Florida.

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21 - Medical Cell and Tissue Biology BMS 6110 John P Aris PhD Rm B1-8 392-1873 [email protected] Reading Ross& Pawlina 5 th Ed Ch 10 Blood&

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