Lab 6 st - Lab 6 Photosynthesis Procedure 1: Pigment...

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Lab 6 Photosynthesis
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Procedure 1: Pigment Separation Pigments: molecules that captures the energy of light Light excites their electrons that begin to transfer through the electron transport system absorb light at characteristic wavelengths pigment isolation : paper chromatography: separates molecules by molecular size, polarity and solubility
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Handle the paper as little as possible- wear gloves Place a line of slurry 1 cm from the bottom edge dry your paper mix before you pipette your sample!!! reapply slurry in exactly the same place and let it dry repeat at least six times, dry between each staple the bottom and top of the paper to create a cylinder put your paper in the jar you have in your table put your cylinder in the jar with the loaded portion down close the jar Allow the liquid to creep up the paper until the solvent front is 0.5-1 cm from the top. Be sure it does not reach the top do not move the jar! it will take 20-40 min
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This note was uploaded on 01/05/2012 for the course BIOL 191 taught by Professor Staff during the Fall '08 term at Boise State.

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Lab 6 st - Lab 6 Photosynthesis Procedure 1: Pigment...

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