Central Dogma - o directs sequence of amino acids o each...

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Central Dogma, Genetic Code using RNA for protein - ribosomes - RNA-protein complexes that make polypeptides o has 2 subunits o RNA acts as main catalytic unit, ribosomal proteins has structural role o protein synthesis occurs at P, A, and E sites ribosomal RNA (rRNA) - type of RNA in ribosomes o provides the site where polypeptides get assembled transfer RNA (tRNA) - transports/positions amino acids messenger RNA (mRNA) - long RNA strands transcribed from DNA reads genetic messages in DNA and produces the proteins that the DNA asks for central dogma - aka gene expression; info passes DNA > RNA > proteins transcription - transfer of info from DNA to RNA o produces mRNA o starts when RNA polymerase binds to promoter binding site o creates complementary transcript (uracil in place of thymine) translation - transfer of info from RNA to protein
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Unformatted text preview: o directs sequence of amino acids o each group of 3 nucleotides codes for an amino acid o rRNA reads the mRNA to make the polypeptide chain genetic code- consists of codons (blocks of information) • same in almost all organisms o supports belief that all organisms have the same root o mitochondria/chloroplasts read code differently • each codon corresponds to a specific amino acid • 3-nucleotide sequence in each codon ( triplet code ) • reading frame- part of genetic code being read by mRNA • removing a single nucleotide or 2 would mess everything up • triplet binding assay - developed by Nirenberg/Leder to see which radioactive amino acid the triplet binded to; tested all 64 possible combinations • mRNA can be transferred from 1 organism to another and still work...
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