Chapter 16 Recombinant DNA and Genetic Engineering

Chapter 16 - Chapter 16 Recombinant DNA and Genetic Engineering I Life and Death on the Threshold of a New Technology A Humans are developing

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Chapter 16 Recombinant DNA and Genetic Engineering I. Life and Death on the Threshold of a New Technology A. Humans are developing technologies that will correct gene mistakes and deficiencies. B. A four-year-old girl with a deficiency of the gene for adenosine deaminase was the first human to be allowed to participate in experimental gene therapy. II. Introduction A. For at least 3 billion years, mutation, crossing over, random gene mixing at fertilization, and hybridizations between species have contributed to the diversity of life on earth. B. By domesticating animals and plants, humans have been manipulating the genetic character of many species for thousands of years. C. Today, we can “engineer” genetic changes through recombinant DNA technology. 1. DNA from different species can be cut, spliced together, and inserted into bacteria which then multiply the DNA necessary for protein production. 2. Genetic engineering has great promise for agriculture, medicine, and industry, but it has also raised ecological, social, and ethical questions. III. Recombinations in Nature: Some Examples A. Transfer of Plasmid Genes 1. Plasmids are circular DNA, or RNA, molecules in bacteria that carry only a few genes and can replicate independently of the single “main” chromosome. 2. The F plasmid is responsible for bacterial conjugation—a transfer of DNA from one cell to another. a. The F+ (donor) has genes for construction of a pilus that attaches to the F– (recipient). b. Then the F+ plasmid DNA unwinds and is copied, and the replicate is transferred to
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This note was uploaded on 01/04/2012 for the course BSC BSC1005 taught by Professor Orlando,rebecca during the Fall '10 term at Broward College.

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Chapter 16 - Chapter 16 Recombinant DNA and Genetic Engineering I Life and Death on the Threshold of a New Technology A Humans are developing

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