DNA Replication - DNA Replication Meselson-Stahl experiment...

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DNA Replication Meselson-Stahl experiment - supported Watson/Crick’s theories on DNA replication DNA replication theories o semiconservative replication - each strand of DNA duplex used when forming new DNA o conservative replication - original DNA duplex remains intact, new DNA has only new molecules o dispersive replication - original DNA gets scattered in new DNA, which contains new/old molecules on each strand bacteria w/ heavier nitrogen (15N) isotope in DNA grown and then transferred to bacteria w/ lighter nitrogen isotope (14N) centrifuge used to determine density of DNA after replication results showed that DNA replicates in a semiconservative way replication process - must be fast/accurate starts at origin, goes bidirectionally towards the terminus replicon - functional unit containing chromosome and origin polymerase - enzyme that synthesizes nucleic acids o 3 main ones: pol I, pol II, pol III o DNA pol II used mainly for DNA repair o DNA pol III - made up of alpha subunit (main catalytic part) and beta subunit
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DNA Replication - DNA Replication Meselson-Stahl experiment...

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