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Unformatted text preview: o DNA separated by size (1st base in sequence found in shortest band, last base in sequence found in longest band) • artificial chromosome- used to clone larger DNA pieces o contained replication origin (to replicate independently of genome) and centromere sequences (for stability) o bacterial artificial chromosomes (BAC) - used for large-scale sequencing, accepts DNA inserts 100-200kb long • clone-by-clone sequencing- physical mapping followed by sequencing o cuts DNA fragments which are each cloned into smaller fragments • shotgun sequencing- sequencing all the clone fragments all at once, uses computer to put together overlaps o assembles consensus sequence from multiple copies of sequenced regions o doesn’t use extra info about genome human genome project- 3.2 gigabase nucleotide sequence in humans • number of genes doesn’t indicate complexity of organism (rice has more genes than humans) • physical map finished on June 26, 2000...
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This note was uploaded on 01/04/2012 for the course BSC BSC1005 taught by Professor Orlando,rebecca during the Fall '10 term at Broward College.
- Fall '10