Retroviruses - Marker genes must have some distinguishable...

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Retroviruses The genetic material of retroviruses is RNA. The retrovirus carries an enzyme called reverse transcriptase , which is capable of creating a DNA copy of the viral RNA. The new DNA produced from the RNA template is called cDNA . DNA synthesis follows the production of cDNA to produce a double-helix. The DNA then becomes incorporated into the host DNA. The new viruses escape the host cell by budding. The AIDS virus (HIV) is an example of a retrovirus. Recombinant DNA Technology Vectors Vectors are DNA used to transfer genes into a host cell. A vector must be capable of self-replicating inside a cell. Marker genes can be used to determine if the gene has been taken up.
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Unformatted text preview: Marker genes must have some distinguishable characteristic. For example if you put a gene that enables an ampicillin resistance on the same vector as the same vector as the gene for human insulin production, then any bacteria that grow on an ampicillin plate will be able to produce insulin. Plasmids The host bacterium takes up the plasmid, which includes the foreign gene. When the bacteria reproduces, the plasmids are also reproduced. The gene is cloned . Shuttle vectors are plasmids that are capable of existing in several different species. They are useful when transferring genes to multicellular organisms....
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This note was uploaded on 01/04/2012 for the course BSC BSC1005 taught by Professor Orlando,rebecca during the Fall '10 term at Broward College.

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