Southern Blotting

Southern Blotting - discussed above Some uses are identification of diseased genes including oncogenes identification of viral infections

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
Southern Blotting Electrophoresis separates DNA by size but does not provide any information about the nucleotide sequence in the DNA. A technique called Southern blotting is used to search for specific base sequences in DNA that has been separated by electrophoresis. It is useful in identifying pathogens and genetic diseases. The DNA is digested using restriction enzymes and separated using gel electrophoresis as described above. The fragments on the gelatin are transferred to a filter by blotting. The filter is treated with an alkaline solution to separate the strands of DNA. A radioactive probe is added to the filter paper and then rinsed off. The probe will hybridize with DNA on the filter only if it is complimentary. It can be visualized using photographic film. Uses for Electrophoresis Electrophoresis requires a large amount of DNA so it is often used in conjunction with PCR
Background image of page 1
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: discussed above. Some uses are identification of diseased genes including oncogenes, identification of viral infections, determining family relationships among individuals, and identifying tissue found at a crime scene. For example, suppose that this procedure is used to identify cells found at a crime scene. Samples A and B (above) came from the scene of the crime and samples C and D came from two different suspects. What can you conclude? Some genetic diseases that can be identified using this procedure are Sickle Cell disease, Huntington’s disease, Duchenne muscular dystrophy. Taxonomists can use this technique to explore evolutionary relationships. Individuals of the same species, while not identical, will be more similar than individuals of different species. The procedure for sequencing and mapping DNA requires RFLP analysis....
View Full Document

This note was uploaded on 01/04/2012 for the course BSC BSC1005 taught by Professor Orlando,rebecca during the Fall '10 term at Broward College.

Ask a homework question - tutors are online