Stages of Genetic Engineering

Stages of Genetic Engineering - Stages of Genetic...

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
Stages of Genetic Engineering DNA cleavage (stage 1) - restriction endonuclease cleaves DNA into fragments produces large number of different fragments different endonucleases >> different fragments gel electrophoresis - procedure that separates fragments based on size recombinant DNA production (stage 2) - DNA fragments inserted into vectors vectors cleaved w/ same restriction endonuclease as DNA cloning (stage 3) - more recombinant DNA created vectors introduced into reproducing cells each reproduced cell contains recombinant DNA screening (stage 4) - most challenging part of any genetics experiment 4-I - preliminary screening of clones o gets rid of cells w/o any vectors, vectors w/o original DNA o uses vector w/ gene for antibiotic resistance o based on presence/absence of a certain phenotype 4-II - finding gene of interest o hybridization - uses complementary nucleic acid ( probe ) to find particular fragment o solution added to denature DNA, allowing radioactive probe to attach
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 2

Stages of Genetic Engineering - Stages of Genetic...

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online