Transcription - Transcription transcription in prokaryotes...

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Transcription transcription in prokaryotes - RNA polymerase - has 5 subunits o 2 a subunits bind regulatory proteins o b' subunit binds DNA template o b subunit binds RNA nucleotide subunits o s subunit starts synthesis, recognizes promoter o template strand (antisense strand) - strand of DNA that’s copied o coding strand (sense strand) - strand of DNA not copied, identical to RNA promoter - sites where transcription starts o short sequence not transcribed by polymerase o -35 sequence - TTGACA, 35 nucleotides from where transcription starts o -10 sequence - TATAAT, 10 nucleotides from where transcription starts initiation - binding of RNA polymerase to promoter o s subunit detects promoter (w/o unwinding DNA), attaches polymerase there o polymerase begins to unwind DNA helix (section about 17 base-pairs long) elongation - uses ATP or GTP to add ribonucleotides o transcription bubble - area containing RNA polymerase, DNA, growing RNA o DNA rewinds after leaving bubble o RNA polymerase can’t proofread, makes more errors than replication
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This note was uploaded on 01/04/2012 for the course BSC BSC1005 taught by Professor Orlando,rebecca during the Fall '10 term at Broward College.

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Transcription - Transcription transcription in prokaryotes...

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