Transgenic Animals

Transgenic Animals - the cell it must become inserted into...

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Transgenic Animals Animal cells generally will not take up plasmids. Other methods such as microinjection must be used. One method has been developed where animal eggs are placed in a mixer with needle-like fragments of silicon carbide. The needles make holes in the cells, allowing DNA to enter. Using this procedure, eggs from fish and several agricultural species have been given the gene for bovine growth hormone, producing larger individuals. Artificial Chromosomes Artificial chromosomes have been created that serve as vectors for introducing large segments of DNA into eukaryotic cells. These chromosomes contain sequences that enable their replication in certain kinds of cells. Viruses Viruses may serve as vectors for transferring DNA into eukaryotic cells. After the DNA enters
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Unformatted text preview: the cell, it must become inserted into the host chromosome. Other Methods of Delivering DNA Electroporation involves using an electric current to create pores in the cell wall and plasma membrane for DNA to enter. It is difficult to create transgenic plants because the cell wall prevents entry of DNA. One solution is to remove the cell wall. These cells (called protoplasts) are then placed in a liquid with foreign DNA. Electroporation is used to make small, temporary holes in the membrane so DNA can pass in. A gene gun propels small gold pellets coated with DNA. The pellets penetrate the cell wall and plasma membrane and enter the cell to deliver their DNA....
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