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Unformatted text preview: cut into four pieces. Other restriction enzymes cut at different sites, some examples are listed below. Enzyme Cutting Site Bam HI GGATCC Hae III GGCC Pst I CTGCAG Hinf I GANTC Sticky Ends Fragments of DNA that has been cut with restriction enzymes have unpaired nucleotides at the ends called sticky ends . All of the fragments will have the same sticky ends. The sticky ends have complimentary bases, so they could rejoin. If the vector and the gene to be cloned are both cut with the same restriction enzyme, they will both have complimentary sticky ends....
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