CHM 410 midterm 2010

CHM 410 midterm 2010 - _ Name CHM 410 / ENV 1410 Analytical...

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__________________________ Name CHM 410 / ENV 1410 Analytical Environmental Chemistry Midterm Exam Fall, 2010 48 marks over 5 questions on 5 pages. Calculators are permitted. Marks for the test are allocated on a ‘mark-a-minute’ basis, so please judge the amount of detail required based on the number of marks allocated for each question.
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1 Question 1. (a) What is the Van Deemter equation for GC applications? (1 mark) (b) What is important to consider when performing large volume LC injections? (1 mark) (c) Draw a functional unit of a weak cation exchange SPE cartridge. (1 mark) (d) What is an exhaustive extraction? (1 mark) (e) When sampling, extracting and analyzing environmental media what types of blanks should you consider and why? (3 mark) (f) Draw a SPLIT GC injection port and describe flows that would result in a split ratio of 50:1. (3 mark)
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2 Question 2. (a) A chromatogram of 3 amino acids run isocratically on a C18 LC column is shown on the right. With a flow rate of 0.3 mL/min and a dead volume of 260 L, what is the k` (retention factor) of the 3 amino acids? Are they appropriately retained? (3 marks) (b) Draw the expected elution order for the 3 amino acids on a HILIC (hydrophilic interaction liquid chromatography) column. (2 marks) 1 2 3 4 5 Response 6 7
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CHM 410 midterm 2010 - _ Name CHM 410 / ENV 1410 Analytical...

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