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L6 - Lecture 6 Separation Theory 1 Chromatography...

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Lecture 6 Separation Theory 1
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Chromatography Definition Separation of components in a mixture based on the differential movement through a porous media under the influence of a mobile phase differential partitioning between the mobile and stationary phases
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Compounds of interest stick to sorbent Solid Phase Extraction Load (sample) Compounds of interest released from sorbent Waste Eluent (solvent) Extract A little bit extreme case Reduce time
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Compounds of interest in a plug Chromatography Load Sample (injection) Differential interaction with stationary phase separates compounds in time and space. Detector Mobile phase Detector Mobile phase
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t 0 t 1 t 2 t 3 t 4 Column elution chromatography t 0 t 1 t 2 t 3 t 4 differential partitioning between the mobile and stationary phases.
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First Chromatography Column toluene sample invented by a Russian botanist Mikhail Tsvet in 1906 during his research on plant pigments glass column packed with shells (calcium carbonate) sorbent elute with a mixture of ether and ethanol chlorophylls and carotenoids Normal phase
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