L13 - Lecture 13 Ion exchange/Ion Chromatography...

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Lecture 13 Ion exchange/Ion Chromatography
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Development of various separation techniques 1903 – Tswett, coined the term chromatography: adsorption chromatogrpahy 1915 R.M Willstatter, German Chemist win Nobel Prize for plant pigments (i.e. chlorophyll) 1931 Richard Kuhn, the first known acceptance of chromatographic methods 1938 Thin Layer chromatography by Russian scientist N.A Izamailov and M.S Shraiber 1941 Liquid-Liquid partition chromatography developed by Archer John, Porter Martin and Richard Laurence Millington Synge 1944 Paper Chromatography one of the most important methods in the development of biotechnology 1945 Gas Chromatography 1 st analytical gas-solid (adsorption) chromatography developed by Fritz Prior 1950 Gas Liquid Chromatography by Martin and Anthony James; Martin won the Nobel Prize in 1952 1966 HPLC named by Csaba Horvath, but only become a popular method until 1970s 1950s Ion-Exchange chromatography declassified this technique 1970s Ion Chromatography was developed by Hamish Small and co-workers at the Dow Chemical company
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Ion exchange/ion chromatography • Ion Exchange Chromatography (IEC) separates biomolecules based on the their net surface charge • Ion Chromatography (IC) more general form of IEC allows separation of ions and polar molecules based on the charge properties of the molecules
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Development of Ion exchange chromatography 1850 Thompson H. and Way J.T. used various clays as an ion exchange material to extract calcium, magnesium, and ammonium ion 1927 the first Zeolite (sodium aluminum silicates) mineral columns were used to remove calcium and magnesium ions from water 1940s Modern Cation-Exchange Chromatography was developed during the wartime Manhattan Project (a code name for the development of the first atomic bomb) - the column was used to concentrate and purify the radioactive elements (e.g., Uranium) that were needed to make an atomic bomb. 1970s Hamish Small and co-workers of Dow Chemical Company developed ion-chromatography (IC) usable for industries - IC uses weaker ionic resins for the stationary phase and a suppressor column was developed to remove background eluent ions for conductivity detection - used for determining low concentrations of ions in water and other environmental studies
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C18 tails Reverse Phase LC H 2 N H 2 N Mobile Phase partition
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• 4 steps – Eluent loading/Conditioning – Sample injection – Separation of samples – Elution
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L13 - Lecture 13 Ion exchange/Ion Chromatography...

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