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L16 - Lecture16 MassSpectrometry1 MS MSIntro (m/z O O O O...

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Lecture 16 Mass Spectrometry 1
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MS Intro MS requires gas phase ions MS requires gas phase ions MS uses magnetic and electric fields to control the path of a compound based on mass to charge ratio (m/z) O O O OH O O O OH OH m/z = 137.11192 m/z = 68.0553 Remember you pH 3 pH 7 MW = 138.1278 calculate mass using the most abundant isotope
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MS Intro MS requires gas phase ions MS requires gas phase ions MS uses magnetic and electric fields to control the path of a compound based on mass to charge ratio (m/z) O O O O NH 2 O NH 3 O NH OH OH NH m/z = 136.1351 3 m/z = N/A pH 4 pH 1 pH 7 2 MW = 137.1438 m/z = 138.1525
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MS Intro MS requires gas phase ions MS requires gas phase ions MS uses magnetic and electric fields to control the path of a compound based on mass to charge ratio (m/z), why? Couple of reasons: 1 Control which ions hit the detector 1. Control which ions hit the detector Quadrupole, magnetic sector 2. Determine how fast an ion hits a detector Time of flight
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