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# L16 - Lecture16 MassSpectrometry1 MS MSIntro (m/z O O O O...

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Lecture 16 Mass Spectrometry 1

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MS Intro MS requires gas phase ions MS requires gas phase ions MS uses magnetic and electric fields to control the path of a compound based on mass to charge ratio (m/z) O O O OH O O O OH OH m/z = 137.11192 m/z = 68.0553 Remember you pH 3 pH 7 MW = 138.1278 calculate mass using the most abundant isotope
MS Intro MS requires gas phase ions MS requires gas phase ions MS uses magnetic and electric fields to control the path of a compound based on mass to charge ratio (m/z) O O O O NH 2 O NH 3 O NH OH OH NH m/z = 136.1351 3 m/z = N/A pH 4 pH 1 pH 7 2 MW = 137.1438 m/z = 138.1525

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MS Intro MS requires gas phase ions MS requires gas phase ions MS uses magnetic and electric fields to control the path of a compound based on mass to charge ratio (m/z), why? Couple of reasons: 1 Control which ions hit the detector 1. Control which ions hit the detector Quadrupole, magnetic sector 2. Determine how fast an ion hits a detector Time of flight