LC-MS Interfaces - Lecture 14 LC-MS Interfaces GC-MS GC MS...

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Lecture 14 LC-MS Interfaces
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GC Computer MS GC-MS
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LC Computer MS LC-MS
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MS Analysis MS Gas phase Ions LC? OH H 3 CH 2 CH 2 C CH 2 CH 2 CH 3 NO 2 O 2 N CF 3 S F F F F F F F O O O
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Condensed Phase Ionization Electrospray Ionization (ESI) Atmospheric Pressure Chemical Ionization (APCI) Atmospheric Pressure Photoionization (APPI) Matrix Assisted Laser Desorption/Ionization (MALDI) Desorption Electrospray Ionization (DESI) Direct Analysis in Real Time (DART) Liquid phase (LC) Solid phase
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PCBs Production started 1920s Env Detection 1968 PFOS Production started 1930s Env Detection 2001 Difference?
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GC-MS and LC-MS ionization are very different GC – Producing ions from gas phase analytes Producing gas phase compounds from charged analytes LC – MS Analysis Necessary for certain important analytes: Biological molecules: Proteins Amino acids/nucleic acids sugars Strong acids without the ability to absorption UV-vis
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Problem solved… John Fenn Electrospray Ionization (ESI)
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Electrospray Ionization (ESI) Gas Nebulized Spray LC Heater ESI Key Features: - Nebulized flow through capillary - Heat applied to evaporate solvent - Capillary (LC) 90 o to Cone (MS) Sample Cone MS Capillary Ionization Chamber 760 torr 10 -6 torr - Atmospheric pressure ion source
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This note was uploaded on 01/05/2012 for the course CHM 410 taught by Professor - during the Fall '11 term at University of Toronto- Toronto.

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LC-MS Interfaces - Lecture 14 LC-MS Interfaces GC-MS GC MS...

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