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6_outline_bio119_cultivation

6_outline_bio119_cultivation - BIO119 II Ways to isolate a...

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Lecture 6 Microbial cultivation and isolation BIO119 I. Isolating a pure culture A.Sample consideration 1. Environmental samples 2. Clinical samples 3. Microbial communities 4. Mixed populations B.What kind of tools to we need? 1. Incubators (shaking/non-shaking) 2. Petri dishes 3. Inoculating loop 4. Bunsen burner 5. Test tubes 6. Sterilization equipment 7. Sterile pipettes 8. Chemicals and water C.Streak plate procedures (Fig. 5.10)
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Lecture 6 Microbial cultivation and isolation BIO119 D.Spread plate procedures (Fig. 5.11A and 5.11B) E. Pour plate (Fig. 5.11C) F. Anaerobic methods (Fig. 5.12) G.Maintaining stock cultures (Fig. 5.13)
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Lecture 6 Microbial cultivation and isolation
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Unformatted text preview: BIO119 II. Ways to isolate a pure culture by enrichment A.First figure out what type of organism you want to isolate. B.Design the growth medium. C.Define the strategy of isolation (see A above) D.Selective culturing on glucose (Fig. 5.14) III. Example #1: The hunt for a haloalkaliphilic archeaon that respires arsenate. A.What is arsenate respiration? B.Why look for this organism (hint: exobiology)? C.What did our team do? D.Were we successful? IV. Example #2: Isolation of a facultative anaerobic arsenate respiring bacterium A.Why did we look for this type of organism? B.Where did we look? C.Our success story....
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