biochem - Stabilizing proteins ELISA Strategy for protein...

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Phi Psi a-helix right-handed -57 -47 parallel B-sheet -119 113 antiparallel B-sheet -139 135 3 10 helix -49 -26 Collagen -51 153 (does not apply to Glycine and Proline) The alpha helix (3.6 13 ) Where n = number of resideues per turn and p = distance the helix rises per turn and d= rise per residue (distance) Silk fibroin is a B-pleated sheet Alpha-keratin are helical, with 5.1A pitch, 3.6 residues per turn Dimer -> protofilament -> microfibril -> macrofibril -> hair
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Unformatted text preview: Stabilizing proteins ELISA Strategy for protein Purification Ionic strength = Salting in Salting out Ion exchange chromatography Gel filtration chromatography Hydrophobic interaction chromatography Affinity Chromatography Electrophoresis (PAGE) 2D gel electrophoresis Ultracentrifugation SDS-PAGE Anion exchange Cation exchange Proteins have a positive net charge below pI, and negative charge above pI...
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This note was uploaded on 01/05/2012 for the course CHEM 3321 taught by Professor Bean during the Spring '11 term at American Jewish University.

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biochem - Stabilizing proteins ELISA Strategy for protein...

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