Liquid Bacterial Inquiry Suggestions

Liquid Bacterial Inquiry Suggestions - Liquid Bacterial...

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
Liquid Bacterial Inquiry Suggestions 1) When ordering liquid media to work with in your inquiry you must ask yourself certain questions: What kind of bacteria am I working with? What is the normal type of media used in scientific experiments for growing my chosen bacteria? How will I transfer the bacteria and treatment while still ensuring that I kept everything sterile? - For several of you the answers to these questions will be E. coli which is typically grown in Trypticase Soy Broth (TSB) – comes in 5mL sterile sample test tubes. You will also need to order sterile loops if working from a plate and sterile pipette tips for the size you will be working with (20uL, 200uL, or 1000uL tips are available). 2) The next question you will have to think about will be how many samples you chose to test. Once you choose the number of samples remember to order enough media to have both positive and negative controls (negative controls will be used to blank the spectrophotometer so you will need one for each treatment you are trying and an additional sample for a positive control to ensure your results are real). 3) How are you going to quantify your results? Using liquid media you will most likely
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 01/06/2012 for the course UGS 303 taught by Professor Foster during the Fall '08 term at University of Texas at Austin.

Page1 / 2

Liquid Bacterial Inquiry Suggestions - Liquid Bacterial...

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online